LOCUS       CHSY3-pGEn2             7869 bp    DNA     circular     20-SEP-2016
FEATURES             Location/Qualifiers
     CDS             129..1108
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_recomb     complement(3839..3867)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    6497..7038
                     /note="WPRE"
     misc_recomb     6459..6481
                     /label=attB2
                     /note="attB2 site"
                     /note="recognized as attB2"
     misc_feature    complement(7045..7259)
                     /note="BGH pA"
     misc_feature    complement(7347..7476)
                     /note="3'-ITR"
     misc_feature    2982..3056
                     /note="TCM signal sequence"
     misc_feature    3060..3083
                     /note="His tag"
     misc_feature    3084..3134
                     /note="AviTag"
     misc_feature    3135..3830
                     /note="Superfolder GFP"
     misc_feature    2976..2981
                     /note="Kozak seq"
     misc_feature    3868..3890
                     /note="TEV site"
     misc_feature    3891..6455
                     /note="OPTIMIZED CHST3 coding region"
     vector          join(6467..7869,1..3857)
                     /source="pGEn2-DEST"
                     /type="Destination Vector"
     clone           3858..6466
                     /source="CHSY3PDONR "
                     /type="Entry Clone"
     source          1..7869
                     /dnas_title="CHSY3-pGEn2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC
     3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC
     3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA
     3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG
     3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG
     3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC
     3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG
     3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC
     3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG
     3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA
     3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA
     3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG
     3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
     3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG
     3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT
     3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc CCTAGAGTGG
     3901 CCGAGCTGAG CGAGAGAAAG AGAAGAGGCT CCAGCCTGTG CAGCTACTAC GGAAGATCTG
     3961 CCGCTGGACC TAGAGCTGGT GCTCAGCAAC CTCTGCCTCA GCCTCAGAGC AGACCCAGAC
     4021 AAGAACAGTC TCCACCTCCT GCCAGACAGG ATCTGCAAGG ACCTCCACTT CCTGAGGCTG
     4081 CCCCTGGCAT CACAAGCTTT AGATCTAGCC CTTGGCAGCA GCCTCCTCCA CTGCAGCAGA
     4141 GAAGAAGAGG CAGAGAACCT GAAGGCGCTA CAGGACTTCC TGGCGCTCCT GCTGCTGAAG
     4201 GCGAGCCTGA AGAGGAAGAT GGCGGAGCTG CTGGCCAGAG AAGAGATGGA AGGCCTGGCA
     4261 GCTCTCACAA TGGAAGCGGA GATGGTGGTG CCGCCGCTCC TTCTGCCAGA CCTAGAGATT
     4321 TTCTGTACGT GGGCGTGATG ACAGCCCAGA AGTACCTGGG ATCTAGAGCC CTGGCTGCCC
     4381 AGAGAACATG GGCCAGATTC ATCCCCGGCA GAGTGGAATT CTTTAGCAGC CAGCAGCCAC
     4441 CTAATGCCGG ACAACCTCCA CCACCACTGC CTGTTATTGC TCTGCCTGGC GTGGACGATA
     4501 GCTACCCTCC TCAGAAAAAG TCCTTCATGA TGATCAAGTA CATGCACGAC CACTACCTGG
     4561 ATAAGTACGA GTGGTTCATG CGGGCCGACG ACGACGTGTA CATCAAGGGC GATAAGCTGG
     4621 AAGAGTTCCT GCGGAGCCTG AACAGCAGCA AGCCTCTGTA TCTGGGACAG ACCGGCCTGG
     4681 GCAATATCGA GGAACTGGGA AAGCTGGGAC TCGAGCCCGG CGAGAATTTC TGTATGGGCG
     4741 GACCTGGCAT GATCTTCAGC CGCGAGGTGC TGAGAAGAAT GGTGCCTCAC ATCGGCGAGT
     4801 GCCTGAGAGA GATGTACACC ACACACGAGG ACGTGGAAGT GGGCAGATGT GTGCGGAGAT
     4861 TTGGCGGCAC ACAGTGCGTG TGGTCTTACG AGATGCAGCA GCTGTTCCAC GAGAACTACG
     4921 AGCACAACCG GAAGGGCTAC ATCCAGGACC TGCACAACTC TAAGATCCAC GCCGCCATCA
     4981 CACTGCACCC TAACAAAAGA CCCGCCTACC AGTACCGGCT GCACAATTAC ATGCTGAGCC
     5041 GGAAGATCAG CGAGCTGCGG TACAGAACCA TTCAGCTGCA TCGGGAAAGC GCCCTGATGT
     5101 CCAAGCTGAG CAACACCGAG GTGTCCAAAG AGGATCAGCA GCTGGGAGTG ATCCCCAGCT
     5161 TCAACCACTT CCAGCCTCGC GAGAGGAACG AAGTGATCGA GTGGGAGTTT CTGACCGGCA
     5221 AGCTGCTGTA CTCTGCCGCC GAAAATCAGC CTCCTAGACA GAGCCTGTCC AGCATCCTGA
     5281 GAACAGCCCT GGACGATACC GTGCTGCAAG TGATGGAAAT GATCAACGAG AACGCCAAGA
     5341 GCCGGGGCAG ACTGATCGAC TTCAAAGAGA TCCAGTACGG CTATCGGAGA GTGAACCCTA
     5401 TGCACGGCGT CGAGTACATC CTGGATCTGC TGCTGCTGTA TAAGCGGCAC AAGGGCAGAA
     5461 AGCTGACCGT GCCTGTTAGA AGGCACGCCT ACCTGCAGCA ACTGTTTAGC AAGCCATTCT
     5521 TCCGCGAAAC CGAAGAACTG GACGTGAACA GCCTGGTGGA ATCCATCAAC AGCGAGACAC
     5581 AGAGCTTCAG CTTCATCAGC AACTCCCTGA AGATCCTGAG CAGCTTCCAG GGCGCCAAAG
     5641 AAATGGGCGG CCACAACGAG AAGAAAGTGC ACATCCTGGT GCCTCTGATC GGCAGATACG
     5701 ACATCTTCCT GCGGTTCATG GAAAACTTCG AGAACATGTG TCTGATCCCC AAGCAGAACG
     5761 TGAAGCTGGT CATCATCCTG TTCAGCAGAG ACAGCGGCCA GGACAGCTCT AAGCACATCG
     5821 AGCTGATCAA GGGCTACCAG AACAAGTACC CCAAGGCCGA GATGACACTG ATCCCTATGA
     5881 AGGGCGAGTT CTCCAGAGGA CTGGGCCTCG AAATGGCCAG CGCTCAGTTC GACAACGACA
     5941 CCCTCCTGCT GTTCTGCGAC GTGGACCTGA TCTTTAGAGA GGATTTCCTG CAGCGGTGCC
     6001 GGGACAATAC CATCCAGGGC CAGCAGGTCT ACTACCCCAT CATCTTTAGC CAGTACGACC
     6061 CCAAAGTGAC CAACGGCGGC AACCCTCCTA CCGACGACTA CTTCATCTTC AGCAAGAAAA
     6121 CCGGCTTTTG GAGAGACTAC GGCTACGGCA TCACCTGTAT CTACAAGAGC GATCTGCTCG
     6181 GCGCTGGCGG CTTCGATACC TCTATTCAAG GCTGGGGCCT CGAGGATGTG GACCTGTACA
     6241 ACAAAGTGAT CCTGTCCGGC CTGCGGCCTT TCAGATCTCA AGAAGTGGGC GTCGTCCACA
     6301 TCTTTCACCC CGTGCACTGC GACCCCAACC TGGATCCTAA GCAGTACAAG ATGTGCCTGG
     6361 GCAGCAAGGC CAGCACCTTT GCCTCTACAA TGCAGCTGGC CGAACTGTGG CTGGAAAAGC
     6421 ACCTGGGCGT CAGATACAAC AGAACCCTGA GCTAGGACCC AGCTTTCTTG TACAAAGTGG
     6481 TTGATAGCTT GGATCCAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT
     6541 TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA
     6601 TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC
     6661 TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC
     6721 TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT
     6781 CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG
     6841 GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC
     6901 CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA
     6961 CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG
     7021 GCCTCTTCCG CGTCTTCGAG ATCTGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT
     7081 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC
     7141 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT
     7201 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAC
     7261 TCGAGTTAAG GGCGAATTGC CGATTAGGAT CTTCCTAGAG CATGGCTACG TAGATAAGTA
     7321 GCATGGCGGG TTAATCATTA ACTACAAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC
     7381 TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT
     7441 TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCCTT AATTAAGAGC TCATGGCGCG
     7501 CCTAGGCCTT GACGGCCTTC CGCCAATTCG CCCTATAGTG AGTCGTATTA CGTCGCGCTC
     7561 ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG
     7621 CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGAAAC
     7681 GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGGA GCGCCCTGTA GCGGCCACTC
     7741 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG
     7801 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT
     7861 ACAATTTAG
//

